GLP-3 (RET) 10mg / 20mg

GLP-3 (RET) is Contexx Research’s coded name for a synthetic, acylated single-chain peptide that acts as an agonist at three related class B G protein-coupled receptors: GIPR, GLP-1R and the glucagon receptor (GCGR). We supply GLP-3 (RET) in Canada as a lyophilized reference material in 10 mg and 20 mg vials; the 10 mg lot has a Janoshik third-party Certificate of Analysis.

GLP-3 (RET) specifications

Research overview

Tri-agonist peptides combine sequence elements of glucagon, GLP-1 and GIP in one molecule so that a single ligand activates all three receptors. Finan and colleagues described the rational design of a monomeric peptide triagonist in 2015, including how they tuned the relative potency at each receptor through iterative sequence changes [1].

Medicinal chemists have refined the concept since. A 2022 Molecular Metabolism study described next-generation GLP-1/GIP/glucagon triple agonists and characterised their receptor potency in cell-based assays alongside studies in mice [2]. The same year, Coskun and colleagues reported the discovery and pharmacology of a fatty-acid-acylated tri-agonist, including in-vitro cAMP potency at each human receptor and receptor-selectivity profiling [3].

In the laboratory, tri-agonist peptides are profiled in GIPR-, GLP-1R- and GCGR-expressing cell lines using cAMP accumulation, β-arrestin recruitment and receptor-internalisation assays. They also serve as reference standards for LC-MS and peptide-stability method development.

Selected references

1. Finan B et al. Nat Med. 2015. PubMed 25485909
2. Knerr PJ et al. Mol Metab. 2022. PubMed 35809773
3. Coskun T et al. Cell Metab. 2022. PubMed 35985340

Storage and handling

GLP-3 (RET) ships as a lyophilized (freeze-dried) powder. Dry material is far more stable than material in solution, so keep it in its dry, sealed state until you need it.

• Store unopened vials of GLP-3 (RET) at −20 °C, dry and protected from light. Let the vial reach room temperature before opening to limit condensation.
• Prepare stock solutions in a solvent suited to your assay, label them with concentration and date, and keep them at 2–8 °C for short-term use.
• For longer storage of solutions, divide them into single-use aliquots and freeze them; avoid repeated freeze–thaw cycles.
• Handle with gloves and eye protection, following your laboratory’s standard chemical-hygiene practice.

See our guide to storing lyophilized peptides in the lab for more detail.

Quality and testing

The 10 mg lot of GLP-3 (RET) has a third-party Certificate of Analysis from Janoshik Analytical, an independent testing laboratory, available through the Check COA button on this page. The report shows the tested identity and purity for that lot. A COA for the 20 mg lot isn’t linked yet; for its testing documentation, contact info@contexxresearch.ca.

We don’t list purity figures in this description; the lot COA is the reference for identity and purity. Researchers should confirm identity and concentration with their own analytical methods where their protocol requires it.

Frequently asked questions

What’s included when I order GLP-3 (RET) 10 mg or 20 mg?

One sealed vial containing 10 mg or 20 mg (as selected) of GLP-3 (RET) as a lyophilized powder. Reconstitution solution is sold separately.

Does GLP-3 (RET) come with a Certificate of Analysis (COA)?

Yes, for the 10 mg vial: a Janoshik third-party COA is available through the Check COA button on this page. A COA for the 20 mg lot isn’t linked yet; for its testing documentation, contact info@contexxresearch.ca.

How fast is shipping in Canada?

Orders are processed Monday to Friday, and most orders ship within 24 hours from Mississauga, Ontario, in secure, tamper-evident packaging. Orders placed on Friday afternoon (after the 12:00 PM ET cut-off) ship on Monday, when Canada Post resumes pickup, or on the next business day if Monday is a statutory holiday. Once your order ships, delivery typically takes 1–3 business days in Ontario and Quebec and 2–6 business days in the rest of Canada, and a tracking number is emailed to you. Orders over $250 CAD shipped within Canada qualify for free standard shipping. See our Shipping Policy for details.

How should GLP-3 (RET) be stored?

Store unopened vials at −20 °C, dry and protected from light. After reconstitution, keep solutions at 2–8 °C for short-term use, or freeze single-use aliquots and avoid repeated freeze–thaw cycles.

What does “research use only” mean?

“Research use only” means GLP-3 (RET) is sold only as a reference material for in-vitro laboratory and analytical research by qualified professionals. It is not for use in humans or animals, not for diagnostic use, and not a drug, natural health product, food, supplement or cosmetic.

Research use only

GLP-3 (RET) is sold by Contexx Research Inc. strictly as a reference material for in-vitro laboratory research and analytical use by qualified professionals. It is not a drug, natural health product, food, dietary supplement or cosmetic. It is not intended for use in humans or animals, for diagnostic purposes, or for ingestion or any other form of administration to a living organism. Nothing on this page describes or implies any effect in humans or animals. Purchasers are responsible for handling, storage and disposal in line with applicable laws and their institution’s policies. By ordering, you confirm that you are purchasing for legitimate laboratory research and agree to our Terms and Conditions.