NAD+ 500mg / 1000mg

NAD+ (β-nicotinamide adenine dinucleotide, oxidised form) is the central redox coenzyme of cellular metabolism and a substrate for sirtuins, PARPs and CD38. Contexx Research supplies NAD+ in Canada as a lyophilized research-grade reagent in 500 mg and 1000 mg vials for biochemistry, enzymology and cell-metabolism research. The 500 mg lot has a Janoshik third-party COA.

NAD+ specifications

Research overview

NAD+ and its reduced form NADH carry electrons in glycolysis, the TCA cycle and oxidative phosphorylation. NAD+ is also consumed as a substrate by signalling enzymes: sirtuin deacylases, poly(ADP-ribose) polymerases (PARPs) and the NADase CD38. Cells maintain their NAD+ pool through de novo synthesis from tryptophan and through salvage pathways using nicotinamide, nicotinamide riboside and nicotinamide mononucleotide. Covarrubias and colleagues review these pathways and how NAD+ levels change in cellular and animal models [1].

A widely cited 2015 review in Science summarises how NAD+-consuming enzymes link cellular energy status to transcription, DNA repair and mitochondrial function in experimental systems [2].

In the laboratory, NAD+ is used as a cofactor in dehydrogenase and sirtuin activity assays, as a substrate for PARP and CD38 enzymology, as a calibration standard for NAD+/NADH quantification by enzymatic cycling or LC-MS, and as a supplement in cell-culture metabolism experiments.

Selected references

1. Covarrubias AJ et al. Nat Rev Mol Cell Biol. 2021. PubMed 33353981
2. Verdin E Science. 2015. PubMed 26785480

Related reading: What is NAD+? A research-only overview of its role in cellular metabolism.

Storage and handling

NAD+ ships as a lyophilized (freeze-dried) solid. Dry material is far more stable than material in solution, so keep it in its dry, sealed state until you need it.

• Store unopened vials of NAD+ at −20 °C, dry and protected from light. This material is hygroscopic, so keep the vial tightly closed and let it reach room temperature before opening to prevent moisture uptake.
• Prepare stock solutions in a solvent suited to your assay, label them with concentration and date, and keep them at 2–8 °C for short-term use.
• For longer storage of solutions, divide them into single-use aliquots and freeze them; avoid repeated freeze–thaw cycles.
• Handle with gloves and eye protection, following your laboratory’s standard chemical-hygiene practice.

Quality and testing

The 500 mg lot of NAD+ has a third-party Certificate of Analysis from Janoshik Analytical, an independent testing laboratory. View the Janoshik COA (also available through the Check COA button on this page). The report shows the tested identity and purity for that lot; check that it matches the product and vial size you ordered. A COA for the 1000 mg lot isn’t linked yet; for its testing documentation, contact info@contexxresearch.ca.

We don’t list purity figures in this description; the lot COA is the reference for identity and purity. Researchers should confirm identity and concentration with their own analytical methods where their protocol requires it.

Frequently asked questions

What’s included when I order NAD+ 500 mg or 1000 mg?

One sealed vial containing 500 mg or 1000 mg (as selected) of NAD+ as a lyophilized powder. Reconstitution solution is sold separately.

Does NAD+ come with a Certificate of Analysis (COA)?

Yes, for the 500 mg vial: that lot has a Janoshik third-party Certificate of Analysis, linked on this page and through the Check COA button. A COA for the 1000 mg lot isn’t linked yet; for its testing documentation, contact info@contexxresearch.ca.

How fast is shipping in Canada?

Orders are processed Monday to Friday, and most orders ship within 24 hours from Mississauga, Ontario, in secure, tamper-evident packaging. Orders placed on Friday afternoon (after the 12:00 PM ET cut-off) ship on Monday, when Canada Post resumes pickup, or on the next business day if Monday is a statutory holiday. Once your order ships, delivery typically takes 1–3 business days in Ontario and Quebec and 2–6 business days in the rest of Canada, and a tracking number is emailed to you. Orders over $250 CAD shipped within Canada qualify for free standard shipping. See our Shipping Policy for details.

How should NAD+ be stored?

Store unopened vials at −20 °C, dry and protected from light. After reconstitution, keep solutions at 2–8 °C for short-term use, or freeze single-use aliquots and avoid repeated freeze–thaw cycles.

What does “research use only” mean?

“Research use only” means NAD+ is sold only as a reference material for in-vitro laboratory and analytical research by qualified professionals. It is not for use in humans or animals, not for diagnostic use, and not a drug, natural health product, food, supplement or cosmetic.

Research use only

NAD+ is sold by Contexx Research Inc. strictly as a reference material for in-vitro laboratory research and analytical use by qualified professionals. It is not a drug, natural health product, food, dietary supplement or cosmetic. It is not intended for use in humans or animals, for diagnostic purposes, or for ingestion or any other form of administration to a living organism. Nothing on this page describes or implies any effect in humans or animals. Purchasers are responsible for handling, storage and disposal in line with applicable laws and their institution’s policies. By ordering, you confirm that you are purchasing for legitimate laboratory research and agree to our Terms and Conditions.